AutoAnalyzer assay for serum alkaline phosphatase activity, with sodium thymolphthalein monophosphate as substrate.
نویسندگان
چکیده
An automated method is described for determining alkaline phosphatase activity in serum, with use of sodium thymolphthalein monophosphate as the substrate. The system makes use of standard AutoAnalyzer components, has a simple flow diagram, and does not involve dialysis. The method has good precision, and the results correlate well with those from the manual method of which it is an adaptation.
منابع مشابه
Ammonium thymolphthalein monophosphate as a new substrate for alkaline and acid phosphatase determinations in serum.
A new substrate, ammonium thymolphthalein monophosphate, is proposed for use in alkaline and acid phosphatase determinations. The ammonium salt shares the advantages of the sodium salt, but is a more stable substrate and results in more stable working reagents. The substrate is buffered at ph 10.1 in diethanolamine for determining alkaline phosphatase activity in 5 mm, and at pH 5.9 in citrate ...
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متن کاملImproved determination of prostatic acid phosphatase (sodium thymolphthalein monophosphate substrate).
We have modified a previously described method for determining acid phosphatase, with thymolphthalein monophosphate as substrate, to increase its sensitivity. We assessed effects of serum on variables influencing acid phosphatase activity as measured by this method. The method is shown to be not completely specific for prostatic acid phosphatase. The importance of standardizing methodology in m...
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Methodological modifications have been made in our “SMA 12/60 AutoAnalyzer” system, to improve the reproducibility and accuracy of six commonly performed procedures. These include glucose with the glucose oxidase method, albumin with the bromcresol green method, and alkaline phosphatase with the sodium thymolphthalein monophosphate method. We have also modified the composition of the reagents u...
متن کاملEffects of substrate concentration on results of determination of prostatic acid phosphatase with thymolphthalein monophosphate.
The relation between concentration of thymolphthalein monophosphate substrate and catalytic activity was investigated for the determination of prostatic acid phosphatase. This study, an extension of previously reported work (Clin. Chem. 27: 1372, 1981), shows that lot-to-lot variation in purity of thymolphthalein monophosphate preparations is reflected in substrate-velocity curves. Plateau regi...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 19 1 شماره
صفحات -
تاریخ انتشار 1973